| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087658 |
| Chromosome: | chromosome 12 |
| Location: | 8127158 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551350 | LAO2 | (1 of 1) 3.5.99.5 - 2-aminomuconate deaminase; Periplasmic L-amino acid oxidase, accessory subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTTCGGCGCCGCGACCTCCTCCATCCTG |
| Internal bar code: | CATCGGCACTGCGTCGCTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1221 |
| LEAP-Seq percent confirming: | 99.6425 |
| LEAP-Seq n confirming: | 17001 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGCACACGTAAACTCAC |
| Suggested primer 2: | CTATGCACCGGCATACACAC |