| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087667 |
| Chromosome: | chromosome 7 |
| Location: | 726401 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317650 | (1 of 1) PTHR11926:SF207 - UDP-GLYCOSYLTRANSFERASE SUPERFAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCATGTGCGACAGAGCAGTACTGGTGA |
| Internal bar code: | TGTCTCGACCCTGAGTGAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 97.2145 |
| LEAP-Seq n confirming: | 1047 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCTGCAAGACAGAAGCC |
| Suggested primer 2: | TGCAACTGTACCTGCCTACG |