| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087679 |
| Chromosome: | chromosome 3 |
| Location: | 5178522 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182350 | (1 of 1) K13176 - THO complex subunit 7 (THOC7) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTCAGAGGTTACATGTGTCCTCTAGCT |
| Internal bar code: | AGAGAGGAGAAAAACCTTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 98.9769 |
| LEAP-Seq n confirming: | 2999 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTTTAACATGGCTGCGGT |
| Suggested primer 2: | GTGCTGGGTCTCGGTATTGT |