Insertion junction: LMJ.RY0402.087679_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.087679
Chromosome:chromosome 3
Location:5178525
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g182350 (1 of 1) K13176 - THO complex subunit 7 (THOC7) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTCTTGATCGGAGCTCCGGGCGCCCGCG
Internal bar code:CCTGACCAGCACCATGTGAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:978
LEAP-Seq percent confirming:99.6322
LEAP-Seq n confirming:18964
LEAP-Seq n nonconfirming:70
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GATTTTAACATGGCTGCGGT
Suggested primer 2:GTGCTGGGTCTCGGTATTGT