| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.087692 |
| Chromosome: | chromosome_1 |
| Location: | 7243868 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g052150 | ANK2,FAP87 | Flagellar Associated Protein with ankyrin repeats | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAGGCCACCGCACACGCAGAGCGGAAGC |
| Internal bar code: | TAGTAGCGTAGGGTTCAGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 99.0318 |
| LEAP-Seq n confirming: | 716 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGTCTTTGCCTGCAACC |
| Suggested primer 2: | ACTCGCTACCAAAGCTTCCA |