Insertion junction: LMJ.RY0402.087704_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.087704
Chromosome:chromosome 6
Location:8127252
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g304913 (1 of 14) PF01476 - LysM domain (LysM) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTTGTGTCTTGGGGCGATGGCGGTGTCG
Internal bar code:TTTGTGAATCTTGGATCATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1107
LEAP-Seq percent confirming:83.8159
LEAP-Seq n confirming:1621
LEAP-Seq n nonconfirming:313
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAATAAGCGGGGCCAAAT
Suggested primer 2:CGTCATTACTGCCGTTGCTA