Insertion junction: LMJ.RY0402.087715_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.087715
Chromosome:chromosome 4
Location:3101880
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g225700 (1 of 1) IPR023401 - Ornithine cyclodeaminase, N-terminal 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCATGCTGACTGCTCTGCCGGTTTTACTG
Internal bar code:GTGGTGTGATCGCGCAGTCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:602
LEAP-Seq percent confirming:25.779
LEAP-Seq n confirming:273
LEAP-Seq n nonconfirming:786
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTTTGCCTCTCTCTGCC
Suggested primer 2:CCAGCCCAAACTAAACCAAA