| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087716 |
| Chromosome: | chromosome 12 |
| Location: | 5132372 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527100 | (1 of 1) IPR002048//IPR003439//IPR003593//IPR027417 - EF-hand domain // ABC transporter-like // AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAAGTTATACTAGCGAGGAGCTACGAGG |
| Internal bar code: | CTAGTTTACTGCTGATGGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1100 |
| LEAP-Seq percent confirming: | 99.7783 |
| LEAP-Seq n confirming: | 450 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAATTGTTCGTGACGGTG |
| Suggested primer 2: | AAGAGGTGGGAGTGTTGTGG |