| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087726 |
| Chromosome: | chromosome 6 |
| Location: | 1750495 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261950 | ANK11 | (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4); Predicted protein with ankyrin repeats | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACTCAATGTGCTAGGGTTCCTCCGCTC |
| Internal bar code: | GGATCTGTCGGCCGGCGCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 95.8446 |
| LEAP-Seq n confirming: | 2837 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGTTCATCCCGAAGCCA |
| Suggested primer 2: | GGTGATAGGACCGCAGTGTT |