| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087746 |
| Chromosome: | chromosome 13 |
| Location: | 1543969 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572900 | THO3 | (1 of 1) 3.4.24.70 - Oligopeptidase A; Oligoendopeptidase | 5'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCTTCTCACGGACCAGCCTTGCCTGCCT |
| Internal bar code: | CGGGCGGAGCCGTTTAGCTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 906 |
| LEAP-Seq percent confirming: | 99.7881 |
| LEAP-Seq n confirming: | 3768 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGGGAAGCTTACATCCTG |
| Suggested primer 2: | GAAGCCAACCTCATTTTCCA |