| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087771 |
| Chromosome: | chromosome 14 |
| Location: | 1328404 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616950 | SELENOT,SELT,SELT1 | (1 of 1) PTHR13544:SF0 - SELENOPROTEIN T; Selenoprotein T | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTCAGCCTCTTGTATTTTGCTGCCGCT |
| Internal bar code: | TACCCCACTCGTGGGCGTTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 99.226 |
| LEAP-Seq n confirming: | 641 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAATTCGGGCTGATTTTC |
| Suggested primer 2: | TTACCCTCCGTTACACAGCC |