Insertion junction: LMJ.RY0402.087787_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.087787
Chromosome:chromosome_8
Location:4151538
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre08.g379900 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GGCGCTGGCGGCGGGGAGCATATCCTCAGA
Internal bar code:ATGAAAGAACGGTAGTGGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:353
LEAP-Seq percent confirming:99.1379
LEAP-Seq n confirming:115
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCCTGTGCTTTCCTGCT
Suggested primer 2:TTGTAACGACAGCTTGCGAC