| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087788 |
| Chromosome: | chromosome 13 |
| Location: | 5090512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607200 | CYG42 | (1 of 5) IPR000104//IPR001054//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTGTGACGCGTGTACCGCGCCGTTGAA |
| Internal bar code: | GGAGCATGAGGGTTTGGCGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 779 |
| LEAP-Seq percent confirming: | 98.4402 |
| LEAP-Seq n confirming: | 1704 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGGCGACTCCTATGGTG |
| Suggested primer 2: | CCCGTTAATGAGACGCAAAT |