Insertion junction: LMJ.RY0402.087812_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.087812
Chromosome:chromosome 6
Location:3326361
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g277350 HDA13 RPD3/HDA1 type histone deacetylase; (1 of 4) K06067 - histone deacetylase 1/2 (HDAC1_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTACTCAAACTCACTTCTAGCAGCGCTT
Internal bar code:AGACTTTCGCGCTATGCATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:419
LEAP-Seq percent confirming:99.6354
LEAP-Seq n confirming:12844
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTACATGTTGTGGGTGGG
Suggested primer 2:GGGAGGGTTGAATCCATTTT