| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087819 |
| Chromosome: | chromosome 14 |
| Location: | 1626256 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618900 | (1 of 2) PF00651//PF07707 - BTB/POZ domain (BTB) // BTB And C-terminal Kelch (BACK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCATGGCCAAGGGAAAGTCCATACAA |
| Internal bar code: | GGGTCTAGGGGTTATTGGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 648 |
| LEAP-Seq percent confirming: | 99.847 |
| LEAP-Seq n confirming: | 1958 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGCGTGTGGTTATTTTT |
| Suggested primer 2: | TATCTCCAGGTTGGGGTCTG |