| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087823 |
| Chromosome: | chromosome 16 |
| Location: | 5784843 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678350 | CAM19 | Calmodulin-like protein; (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGGGCTGGGATGGCGGGGTAGGCCGTAG |
| Internal bar code: | ATCTCAGATCGCAGCTGAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 89.5522 |
| LEAP-Seq n confirming: | 420 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGGATACAGAGCAGCCT |
| Suggested primer 2: | CTCGCTTCCTTATGCCACTC |