| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087848 |
| Chromosome: | chromosome 4 |
| Location: | 3237199 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226600 | ARS12 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTTGGGGCGTGTGGGTGAAGGGTGGGA |
| Internal bar code: | GGCGGCCTGGGACTTCATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 89.0756 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTACCCAAACCCTTTCT |
| Suggested primer 2: | TTCCCCAAGCAGTACCAGAC |