Insertion junction: LMJ.RY0402.087869_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.087869
Chromosome:chromosome 10
Location:465100
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g420950 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTCGCCTTTTGCCCCAACTAACCTTCGC
Internal bar code:CGGACGGGCTCAGTGCCACCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:228
LEAP-Seq percent confirming:89.7706
LEAP-Seq n confirming:1448
LEAP-Seq n nonconfirming:165
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCAAAAGCTTGGCATGAG
Suggested primer 2:CTCCAGATGCAGGTCAGACA