Insertion junction: LMJ.RY0402.087871_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.087871
Chromosome:chromosome 6
Location:3512459
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278107 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCCTTTCTAGCTTTAAAGGAAAGACTTT
Internal bar code:TGCTCCTAGTGATAAGAACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:435
LEAP-Seq percent confirming:99.4955
LEAP-Seq n confirming:986
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGAGTCCATTTGTTGGC
Suggested primer 2:TTGATAGAAGCGTTCGGGAC