| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087871 |
| Chromosome: | chromosome 14 |
| Location: | 688912 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612500 | (1 of 1) K05954 - protein farnesyltransferase subunit beta (FNTB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCGTGGAGCGTGCTGTTGCCGCGGAGC |
| Internal bar code: | AAGCCGGGGGATCGATGTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 98.8214 |
| LEAP-Seq n confirming: | 8385 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCAACGTGGTGGATGAC |
| Suggested primer 2: | ATTAGTTGCAATCGGCCAAC |