| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087893 |
| Chromosome: | chromosome 9 |
| Location: | 7653592 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415300 | GT90-19,GT90F19 | GT90 family protein 19; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTTGCATGAATGGCACGCGTCCGGACA |
| Internal bar code: | GGTGACGCGGTTCGGTGCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 98.8182 |
| LEAP-Seq n confirming: | 1087 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCTAGCCGCATCCTGTT |
| Suggested primer 2: | AGGAGAGGTGTCGCTGAAAA |