| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087896 |
| Chromosome: | chromosome 16 |
| Location: | 4596926 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687850 | PTK22 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype; Protein of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATGGGTGGCCCTAGGCGTGTCTTGCAG |
| Internal bar code: | CCTTCTGCGCAGCTTAATTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 63 |
| LEAP-Seq percent confirming: | 55.1948 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTGCAGGTGGAAGAAGC |
| Suggested primer 2: | GGAGTCGGTGTGACCAAACT |