| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087904 |
| Chromosome: | chromosome 3 |
| Location: | 1181237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149450 | (1 of 1) IPR006037//IPR016040 - Regulator of K+ conductance, C-terminal // NAD(P)-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTCGCTGCCCCAGCGAGCGGCCAGCAT |
| Internal bar code: | CGGGTGAGCATCGGTAAACCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 19.5652 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACTGCTGCTGCTAGCGTG |
| Suggested primer 2: | ACTAACCCCACTGTGTTGCC |