| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087907 |
| Chromosome: | chromosome 13 |
| Location: | 2714582 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582270 | FFT3 | putative fructan fructosyltransferase; (1 of 1) IPR001362//IPR013148//IPR023296 - Glycoside hydrolase, family 32 // Glycosyl hydrolase family 32, N-terminal // Glycosyl hydrolase, five-bladed beta-propellor domain | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCCCATGCTTCCTTCCCCTTTGCGTAT |
| Internal bar code: | CTTGGTAAAAGGTATTCCCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1272 |
| LEAP-Seq percent confirming: | 99.6248 |
| LEAP-Seq n confirming: | 2124 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACGG |
| Suggested primer 2: | TTCCCAACAAACGGCTAAAC |