| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087923 |
| Chromosome: | chromosome 12 |
| Location: | 8705534 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546100 | FAP125,KIN14-2 | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Flagellar Associated Protein 125 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGTGGGCGCTGACTCAGCGGATGGCT |
| Internal bar code: | ACTCGAAGTTAAGCCCTTCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 99.8742 |
| LEAP-Seq n confirming: | 4762 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCGCTTGTGAGCATACCT |
| Suggested primer 2: | GTCTCTGTGGTGAGTGGGGT |