| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087924 |
| Chromosome: | chromosome 3 |
| Location: | 7700382 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203700 | DEGO1,DEG12 | DegP-type protease-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCCTAGGCATGGCGTGTTCATGCGGAA |
| Internal bar code: | CAGGTGCGGGTGTCAGGTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCGCCATGGTATCGTCT |
| Suggested primer 2: | CCAAAGATGGATCAGGCAGT |