| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087934 |
| Chromosome: | chromosome 11 |
| Location: | 625365 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467613 | (1 of 1) IPR000742//IPR001881 - EGF-like domain // EGF-like calcium-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGCACAGCGACTTGGCCGCATCGCTCC |
| Internal bar code: | GCTCTATGGTATGTCTGGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 97.3783 |
| LEAP-Seq n confirming: | 1300 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCCAGCTTCTCCCTCAC |
| Suggested primer 2: | TCCTGCGAAGGGATATTTTG |