| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087936 |
| Chromosome: | chromosome 3 |
| Location: | 6343411 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193700 | (1 of 1) PTHR10992//PTHR10992:SF749 - ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCACCCGCCAGCCAGCCCACATCGCAA |
| Internal bar code: | AGCAACGCGATGGGCTGCTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 99.2627 |
| LEAP-Seq n confirming: | 2558 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTGCGTCTATTGGGTTT |
| Suggested primer 2: | CCAAGAGTTCGACCCACTTC |