| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.087953 |
| Chromosome: | chromosome 5 |
| Location: | 3375685 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240900 | (1 of 1) IPR000001//IPR024079 - Kringle // Metallopeptidase, catalytic domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGGTAGACGGCAGACCCATCGGGGTGG |
| Internal bar code: | TGTTTAGTTGCTCGGAACGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTCTACCATGTCCTTGC |
| Suggested primer 2: | CGCAGCAAACTACTGCTGAC |