| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087981 |
| Chromosome: | chromosome 3 |
| Location: | 155785 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144144 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATATGGAGGGACTGAGCACGTGGGAAA |
| Internal bar code: | GGTTTCACGGAGTTCTTCTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 98.6075 |
| LEAP-Seq n confirming: | 3399 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACAGTGCACAAGGCTTC |
| Suggested primer 2: | GAAAGCGAAGCTTACATGGC |