| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.087983 |
| Chromosome: | chromosome 1 |
| Location: | 758839 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003950 | (1 of 28) PTHR31600:SF2 - TINY MACROCYSTS PROTEIN B-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTGTTTTTAACCCTATGCACGGCATGCT |
| Internal bar code: | CTTTGAGAACTCGCCAGGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 94.0926 |
| LEAP-Seq n confirming: | 21662 |
| LEAP-Seq n nonconfirming: | 1360 |
| LEAP-Seq n unique pos: | 113 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAAGACCAAGCAGAAGG |
| Suggested primer 2: | GTAGGCAAGTCCCGTCATGT |