| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.087995 |
| Chromosome: | chromosome 1 |
| Location: | 7253744 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052300 | E2F1 | (1 of 1) K06620 - transcription factor E2F3 (E2F3); Transcription factor, E2F and DP-related | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTCTCCCCACTCCATGACTTGCATCTC |
| Internal bar code: | GGAATAATGAATGTCTTGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 99.6283 |
| LEAP-Seq n confirming: | 2144 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTGGGGTACTCAAGGAG |
| Suggested primer 2: | TTAGGGAGGCAGGAAAAGGT |