| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.087995 |
| Chromosome: | chromosome 6 |
| Location: | 7766369 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGCGCAGGAGAACAGGGGCACCCCGGT |
| Internal bar code: | CAATCGCAGGTAAGGTCTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 130 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACTGACGTACCCTGTCC |
| Suggested primer 2: | CTTGAGGGGGCATACTTGAA |