| Insertion cassette: | CIB1 | 
| Side of cassette: | 3' | 
| Strand: | + | 
| Strain: | LMJ.RY0402.087996 | 
| Chromosome: | chromosome 2 | 
| Location: | 5011637 | 
| Confidence (%): | 73 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre02.g104250 | intron | 
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGGGCTCCCGTCGCATTTTGTGAGGGT | 
| Internal bar code: | CAATAGGGCGAATCCACGAACC | 
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 | 
| LEAP-Seq percent confirming: | 97.3269 | 
| LEAP-Seq n confirming: | 1602 | 
| LEAP-Seq n nonconfirming: | 44 | 
| LEAP-Seq n unique pos: | 9 | 
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCTAGGTTACCACGTCC | 
| Suggested primer 2: | AGACCCTGTTCGCTCTCAAA |