Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.087997 |
Chromosome: | chromosome_13 |
Location: | 3741498 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589450 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAGTGAAAGCGAGTTGTGTTTTGCTTAAAG |
Internal bar code: | CCGAGGGATTCGAATTGCCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 283 |
LEAP-Seq percent confirming: | 99.7055 |
LEAP-Seq n confirming: | 2031 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACATGCCTGGTTGTAAGCC |
Suggested primer 2: | GAGGTAGCTGAACTCCACCG |