| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088002 |
| Chromosome: | chromosome 3 |
| Location: | 5394438 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184700 | (1 of 1) PTHR31810//PTHR31810:SF6 - FAMILY NOT NAMED // METACASPASE-4-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGATGGGGGGGGGACGGGTGTGTGTCA |
| Internal bar code: | AGGAGAGGGGGCAGATAACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1174 |
| LEAP-Seq percent confirming: | 99.1021 |
| LEAP-Seq n confirming: | 883 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGCTACCGGTGCCTTAC |
| Suggested primer 2: | CGGACCTGACCATTCTGATT |