| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088039 |
| Chromosome: | chromosome 4 |
| Location: | 1562054 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213500 | TMG8 | (1 of 1) PTHR23417//PTHR23417:SF18 - 3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE // SUBFAMILY NOT NAMED; tRNA (guanine-N7)-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCGCATTTCAGCCATGGTTACTGTATC |
| Internal bar code: | GCGTCAGACGGTTGATCTACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 98.836 |
| LEAP-Seq n confirming: | 18001 |
| LEAP-Seq n nonconfirming: | 212 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGCTCGAAGGATGACA |
| Suggested primer 2: | CCTCTTTCGCCATCTTCTTG |