| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088052 |
| Chromosome: | chromosome 14 |
| Location: | 2779155 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627200 | (1 of 4) PTHR13140//PTHR13140:SF289 - MYOSIN // UNCONVENTIONAL MYOSIN-XIX | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGATCTTTCTGAAAGAGCACGGCCCCCA |
| Internal bar code: | GTGCTTTCGTCCGCGCTCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1138 |
| LEAP-Seq percent confirming: | 64.3846 |
| LEAP-Seq n confirming: | 3395 |
| LEAP-Seq n nonconfirming: | 1878 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAACCGCACCTTCTACCCA |
| Suggested primer 2: | TATGCCTGATTGAGTGCAGC |