Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.088057 |
Chromosome: | chromosome 16 |
Location: | 955447 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g648700 | (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGATTGGGCTGGTATATACAACTCCCCCC |
Internal bar code: | GGGGGGTACAGTCGGGCAATTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 123 |
LEAP-Seq percent confirming: | 94.2478 |
LEAP-Seq n confirming: | 213 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGACGACATGAAGTTGGGG |
Suggested primer 2: | AGTCCCGTGCACATACACAA |