| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088071 |
| Chromosome: | chromosome 13 |
| Location: | 4872850 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605800 | (1 of 1) PTHR11544:SF59 - COLD SHOCK-LIKE PROTEIN CSPF-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGGGAATGCAACCTGGCCAGATGCCG |
| Internal bar code: | AAGGGGTTCCGTCTTTGCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 99.7283 |
| LEAP-Seq n confirming: | 1101 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTTGTTGTTGCTGCT |
| Suggested primer 2: | GGCTGCTGCTAACATGAACA |