| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088076 |
| Chromosome: | chromosome 3 |
| Location: | 2865072 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162701 | SRH5 | SNF2-related DNA/RNA helicase; (1 of 1) 3.6.4.11 - Nucleoplasmin ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCTGCTGCTCACAGGTGCGTGCGCAGC |
| Internal bar code: | TCCGTGGAATGAGAGGCGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 89.702 |
| LEAP-Seq n confirming: | 2709 |
| LEAP-Seq n nonconfirming: | 311 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATAAGAGGTGCGGTTGG |
| Suggested primer 2: | GGGGGTTACAAGCACGACTA |