Insertion junction: LMJ.RY0402.088077_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.088077
Chromosome:chromosome 16
Location:4598406
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g687800 PTK22 (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Protein tyrosine kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCCAGCCCGTGCACACCACCGTGCCAT
Internal bar code:ACTGGGAGAGGGCTGAGTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:340
LEAP-Seq percent confirming:88.0337
LEAP-Seq n confirming:2928
LEAP-Seq n nonconfirming:398
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACATTCATTCGCTAAACCGC
Suggested primer 2:ATACAGAACCTGTACGGCGG