| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088099 |
| Chromosome: | chromosome 8 |
| Location: | 512404 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359000 | (1 of 32) IPR006502 - Protein of unknown function PDDEXK-like | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATAGATCGATGTCGCAGCAACAGCAGC |
| Internal bar code: | GAGTAGGCGTGATCTTCGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 99.7409 |
| LEAP-Seq n confirming: | 385 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTAAATACATTGGCGGCG |
| Suggested primer 2: | ACTCTGGTAATGGTGGTGGC |