| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088109 |
| Chromosome: | chromosome_2 |
| Location: | 5993552 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g112200 | sense | 5'UTR_intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GACAGCCGGGTCAGGCACGGGGCACGGCCG |
| Internal bar code: | GCTCGACGAACCTCTTAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 99.9054 |
| LEAP-Seq n confirming: | 4223 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCGCTCTCATTTTAACTC |
| Suggested primer 2: | AGGTCCTTCACCAGGTCCTT |