| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088134 |
| Chromosome: | chromosome 6 |
| Location: | 3881708 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278177 | DNL6,LIG6 | (1 of 1) PTHR10459:SF44 - ANKYRIN REPEAT-CONTAINING PROTEIN; DNA ligase 6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTCTCGTCCACCAGGCTGCGGAAGCGC |
| Internal bar code: | ATTGCAACGGGTTTATTTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 87.3053 |
| LEAP-Seq n confirming: | 1850 |
| LEAP-Seq n nonconfirming: | 269 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGATCAGCACCACAACAT |
| Suggested primer 2: | TACGAGGTGAGGGTTTGACC |