| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088152 |
| Chromosome: | chromosome 13 |
| Location: | 2735623 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582476 | (1 of 1) K01112 - phosphohistidine phosphatase (PHPT1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATGAAATAAATGGAATCGTACAATGTT |
| Internal bar code: | TTCGATGTGGTGCTGGCGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 408 |
| LEAP-Seq percent confirming: | 99.277 |
| LEAP-Seq n confirming: | 2197 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATTAGCCACGTCCACTC |
| Suggested primer 2: | ATTGAGGCTAACAACCCACG |