| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088170 |
| Chromosome: | chromosome 2 |
| Location: | 5113640 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105000 | (1 of 3) PTHR11207//PTHR11207:SF7 - RIBONUCLEASE III // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACTCGGCCGCGCTGGTCCGCAAGCCCAC |
| Internal bar code: | GGCGCCGGCGCTTCTTACGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 94.8718 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGGCTCAAGTAGGACGG |
| Suggested primer 2: | TCGGGTATCGAAACTTCGTC |