Insertion junction: LMJ.RY0402.088179_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.088179
Chromosome:chromosome 2
Location:285369
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g075050 PTA1 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGACTCCGCCGCAGAACACTGCTGACCC
Internal bar code:GGGGTTTGCTTAAAGTATTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1394
LEAP-Seq percent confirming:99.8999
LEAP-Seq n confirming:1997
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCGCTTGATATTCAGCTT
Suggested primer 2:CGGTACCCTGATGTCGAGTT