| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088191 |
| Chromosome: | chromosome_6 |
| Location: | 1388467 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g258800 | CWP2,GP2 | Hydroxyproline-rich glycoprotein component of the outer cell wal | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCTTTGTTAAGCCCCGGTGCGCAGACGCC |
| Internal bar code: | ACGGTATGCGGGTCCAAGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 98.7578 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCTCAAGGCTGTGTTCC |
| Suggested primer 2: | ACATTTTGGGGGTGAATGAA |