| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088197 |
| Chromosome: | chromosome 3 |
| Location: | 8568750 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202337 | MOT28 | (1 of 2) PF14753 - Domain of unknown function (DUF4475) (DUF4475); Predicted protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTCCGGCTATCGTTGACGCCTGTTGTC |
| Internal bar code: | GTGAGGGGTTATTTGGCCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 98.852 |
| LEAP-Seq n confirming: | 3272 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTGTGAGAGCTCGTTCC |
| Suggested primer 2: | CGTATGCAAGAAGGGGTTGT |